Perfusion Tray

Syringe pump system

The pumping system on each tray can be composed of one (or multiple) pair of oscillating syringe pumps, which exchange nutrient medium between two syringes through the culture dish. The pumping system allows automatic regulation and manual adjustment of medium flow, as well as motor position using control buttons and a Wi-Fi connected web interface

Build	Aluminium
Charging	External supply (from incubator housing if used in combination)
Pump	Stepper motor driven syringe pump system
Syringes	2x20 ml Braun Omnifix with Luer lock (or dimensionally equivalent)
Flow	0.05 - 2 mL/min (± 0.01 ml/min)
Tubing	Cell culture silicone (biocompatible, autoclavable)
connections	Luer-lock (PP)
Dead volume	<5 ml
Monitoring/Control	Digital display, control buttons, WiFi via web interface
Control parameters	Flow rate, motor position
Sensors	Motor limit switches, temperature

"De-bubbler" (bubble trap)

Two bubble traps/debubblers are installed per tray in between the culture dish and each syringe pump to prevent possible air bubbles from entering the tissue samples during medium flow from either side. As a consequence, each membrane based unit also allows gas exchange by diffusion.

Build	Custom construction (PEEK/POM/...)
Membrane	PTFE, 0.2 um porosity
Maximum flow rate	2ml/min (both directions)
Tubing connections	Luer-lock
Other	Autoclavable, leak-proof

Custom tissue platform

The custom-made culture dish is adapted for the perfusable New Harvest tissue samples and fulfills the following functions:

• Allow simple inserting and exchange of samples,

• attaching samples to the perfusion system via catheter, while being leak-proof.

• The samples are kept in place, however, the container will allow contractions of the sample.

• The housing is autoclavable, suitable for reuse and allow assembly without additional tools. In addition, the cover will contain a glass window made from standard microscope slides to allow observation without disturbing the samples.

Build	Custom construction (PEEK/POM/...)
Sample container	Holding sample in place while allowing contraction and extension in Y and Z directions. Sample insert/exchange in 2 steps without additional tools.
Perfusion	Leak-proof catheter (peripheral venous type) insert/fastening in 2 steps without additional tools, luer-lock connectivity.
Other features	Autoclavable, transparent glass window (microscope slide)